An Introduction to Recombinant Protein Production, Purification, and Quality Assessment
Sponsored by the Interreg SK-AT project “CAPSID”
[03.12.2019 – 04.12.2019, Vienna] This workshop will provide a basic introduction into recombinant protein production in eukaryotic expression systems, protein purification, including protein complexes, secreted proteins and viruses and will also discuss important aspects of protein quality assessment. The workshop is intended for Masters’ and PhD students, postdocs and other researchers in the Vienna-Bratislava cross-border region.
- Eva Kutejova, Slovak Academy of Sciences
- Clemens Plaschka, Research Institute of Molecular Pathology
- Kim Remans, European Molecular Biology Laboratory
- Arthur Sedivy, Vienna BioCenter Core Facilities
- Ondrej Vanek, Charles University Prague
- Ivana Nemčovičová, Biomedical Research Center
- Marta Šiborová, Central European Institute of Technolog
- Antonio Sponga, University of Vienna
CAPSID Workshop Registration
Registration is closed.
Capsid Seminar Series
[06.11/07.11.2019 Bratislava/Vienna] In the framework of Interreg project CAPSID, the second talk for our seminar series on virology in the cross-border region took place.
Bertrand Raynal was emphasizing two important topics in modern structural biology: sample quality control and data validation. In his position as expert in biophysicalcharacterization in the core facility at Institute Pasteur (Paris) he established minimum quality criteria for purified recombinant proteins and implemented them as services to assess the actual state of the samples used for biochemical/biophysical experiments. This is one important step towards tackling the reproducibility problem biological sciences face nowadays. The second part of the talk described how one can use hydrodynamic information about protein (complexes) measured on benchtop instrument can cross validatehigh resolution structural information (e.g. from X-ray crystallography, cryo-EM but also SAXS experiments). Finally he developed a software tool that can de-novo generate protein(complex) shape information from just hydrodynamic information measured in benchtopinstruments (e.g. SEC-MALLS, viscometer, AUC, DLS).
International conference on virology in SK-AT cross-border region!
[15.02.2019 Bratislava] CAPSID project members Peggy Stolt-Bergner (VBCF), Ivana Nemcovicova (BMC SAV) and Martin Puchinger (VBCF) participated in the Joint Virology Conference and the 1st SK-AT Structural Biology Meeting 2019 in Bratislava from 13th to 15th February 2019.
The conference brought together international scientists working on all fields of virology research from virus ecology and evolution, through epidemiology and virus-host interactions to structural and biochemical virology. This conference increased the international awareness of the cutting edge virology research in the cross-border region, served to stimulate scientific exchange and created opportunities for new collaborations among the participants.
CAPSID Seminar Series!
[11.12.2018 Bratislava/Vienna] In the framework of Interreg project CAPSID, the seminar series on virology in the cross-border region has started. Prof. Ettrich from Larking University Miami, (US) gave two exciting talks about modelling and simulating cation translocation in human ORAI channels. His work combines experimental and theoretical approaches to elucidate this important means of signal transduction.
Kick-Off Meeting for Interreg project CAPSID!
[17.07.2018 Vienna] On 17th July, the project partners VBCF and BMC met in Vienna to kick-off the Interreg project CAPSID. The project is dedicated to increase the international visibility of the biomedical research landscape in the Slovak-Austrian border region. With a total of 11 project partners, Vienna Biocenter Core Facilities (VBCF) in Vienna and the Biomedical Research Center of the Slovak Academy of Sciences in Bratislava have joined forces to improve the scientific communication in the region and the international visibility of the cross-border virological and biomedical research community.
CAPSID is co-financed by the European Regional Development Fund (ERDF) under the Interreg V-A Slovakia-Austria program.