Welcome to the CAPSID Wiki page.
This is an interactive database for protocols on protein expression and purification. Protocols are free to download and use. If you have suggestions for improvements or corrections, please use the comment function below.
Protein Expression and Purification Protocols
| SOP developed by project partner | Title of SOP | Application | Last updated on |
|---|---|---|---|
| VBCF | Manual Magnetic Bead IMAC Screen Protocol | Test expression in E. coli and purification using magnetic IMAC beads of target constructs | April, 27th 2021 |
| VBCF | Transient Transfection of EXPRES2 cells in 24-well plate | Transiently transfect Expres2 cells in 24-well format for production of secreted recombinant proteins | April, 27th 2021 |
| VBCF | E.coli small-scale expression and purification | Test expression and affinity purification of several constructs in parallel | April, 27th 2021 |
| VBCF | HEK293 FreeStyle – PiggyBac stable cell lines | Inducible recombinant expression in mammalian system in form of stable cell line generation | April, 27th 2021 |
| BMC | Refolding by dialysis from insoluble fraction of bead mill-lysed Ecoli cells | Refolding by dialysis from insoluble fraction of bead mill-lysed Ecoli cells | June, 7th 2021 |
| BMC | Refolding by dilution from insoluble fraction of microfluidizer-lysed Ecoli cells | Refolding by dilution from insoluble fraction of microfluidizer-lysed Ecoli cells | June, 7th 2021 |
| BMC | On-column refolding from Ecoli inclusion bodies | On-column refolding from Ecoli inclusion bodies | June, 7th 2021 |
| BMC | Preparing a selenomethionyl-labeled protein in BV-Sf9 insect cells | Preparing a selenomethionyl-labeled protein in BV-Sf9 insect cells | June, 7th 2021 |
| BMC | Preparing a selenomethionyl-labeled protein in Ecoli cells | Preparing a selenomethionyl-labeled protein in Ecoli cells | June, 7th 2021 |