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This is an interactive database for protocols on protein expression and purification. Protocols are free to download and use. If you have suggestions for improvements or corrections, please use the comment function below.

Protein Expression and Purification Protocols

SOP developed by project partnerTitle of SOPApplicationLast updated on
VBCFManual Magnetic Bead IMAC Screen ProtocolTest expression in E. coli and purification using magnetic IMAC beads of target constructsApril, 27th 2021
VBCFTransient Transfection of EXPRES2 cells in 24-well plateTransiently transfect Expres2 cells in 24-well format for production of secreted recombinant proteinsApril, 27th 2021
VBCFE.coli small-scale expression and purificationTest expression and affinity purification of several constructs in parallel
April, 27th 2021
VBCFHEK293 FreeStyle – PiggyBac stable cell linesInducible recombinant expression in mammalian system in form of stable cell line generationApril, 27th 2021
BMCRefolding by dialysis from insoluble fraction of bead mill-lysed Ecoli cellsRefolding by dialysis from insoluble fraction
of bead mill-lysed Ecoli cells
June, 7th 2021
BMCRefolding by dilution from insoluble fraction of microfluidizer-lysed Ecoli cellsRefolding by dilution from insoluble fraction
of microfluidizer-lysed Ecoli cells
June, 7th 2021
BMC
On-column refolding from Ecoli inclusion bodiesOn-column refolding from Ecoli inclusion bodiesJune, 7th 2021
BMCPreparing a selenomethionyl-labeled protein in BV-Sf9 insect cellsPreparing a selenomethionyl-labeled protein in BV-Sf9 insect cellsJune, 7th 2021
BMCPreparing a selenomethionyl-labeled protein in Ecoli cellsPreparing a selenomethionyl-labeled protein in Ecoli cellsJune, 7th 2021


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